Fluorescence Microscopy and Fluorescent Probes

Written By J. Slavík
Fluorescence Microscopy and Fluorescent Probes
  • Publsiher : Springer Science & Business Media
  • Release : 11 November 2013
  • ISBN : 1489918663
  • Pages : 306 pages
  • Rating : 4/5 from 21 reviews
GET THIS BOOKFluorescence Microscopy and Fluorescent Probes


Read or download book entitled Fluorescence Microscopy and Fluorescent Probes written by J. Slavík which was release on 11 November 2013, this book published by Springer Science & Business Media. Available in PDF, EPUB and Kindle Format. Book excerpt: Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Fluorescence Microscopy and Fluorescent Probes

Fluorescence Microscopy and Fluorescent Probes
  • Author : J. Slavík
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-11-11
  • Total pages : 306
  • ISBN : 1489918663
GET BOOK

Summary : Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be ...

Fundamentals of Fluorescence Microscopy

Fundamentals of Fluorescence Microscopy
  • Author : Partha Pratim Mondal,Alberto Diaspro
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-12-12
  • Total pages : 218
  • ISBN : 1489918663
GET BOOK

Summary : This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular ...

Fluorescence Microscopy

Fluorescence Microscopy
  • Author : Ulrich Kubitscheck
  • Publisher : John Wiley & Sons
  • Release Date : 2017-05-08
  • Total pages : 504
  • ISBN : 1489918663
GET BOOK

Summary : While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy ...

Fluorescence Microscopy

Fluorescence Microscopy
  • Author : F. W. D. Rost
  • Publisher : Cambridge University Press
  • Release Date : 1992
  • Total pages : 473
  • ISBN : 1489918663
GET BOOK

Summary : Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with ...

Introduction to Confocal Fluorescence Microscopy

Introduction to Confocal Fluorescence Microscopy
  • Author : Michiel Muller
  • Publisher : SPIE Press
  • Release Date : 2006
  • Total pages : 119
  • ISBN : 1489918663
GET BOOK

Summary : This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and ...

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy
  • Author : Alberto Diaspro
  • Publisher : CRC Press
  • Release Date : 2010-04-26
  • Total pages : 448
  • ISBN : 1489918663
GET BOOK

Summary : "Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods ...

Quantitative Fluorescence Microscopy

Quantitative Fluorescence Microscopy
  • Author : F. W. D. Rost,Rost
  • Publisher : Cambridge University Press
  • Release Date : 1991-07-26
  • Total pages : 236
  • ISBN : 1489918663
GET BOOK

Summary : Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of ...

Fluorescence Microscopy in Life Sciences

Fluorescence Microscopy in Life Sciences
  • Author : Juan Carlos Stockert ,Alfonso Blazquez-Castro
  • Publisher : Bentham Science Publishers
  • Release Date : 2017-12-15
  • Total pages : 751
  • ISBN : 1489918663
GET BOOK

Summary : Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms ...

Optical Fluorescence Microscopy

Optical Fluorescence Microscopy
  • Author : Alberto Diaspro
  • Publisher : Springer Science & Business Media
  • Release Date : 2010-11-25
  • Total pages : 244
  • ISBN : 1489918663
GET BOOK

Summary : In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array ...

Fluorescence Microscopy of Living Cells in Culture Part A

Fluorescence Microscopy of Living Cells in Culture  Part A
  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 1989-02-01
  • Total pages : 332
  • ISBN : 1489918663
GET BOOK

Summary : Fluorescence Microscopy of Living Cells in Culture, Part A...

Fluorescence Microscopy of Living Cells in Culture Part B

Fluorescence Microscopy of Living Cells in Culture  Part B
  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 1989-04-01
  • Total pages : 502
  • ISBN : 1489918663
GET BOOK

Summary : Fluorescence Microscopy of Living Cells in Culture, Part B...

Protein Localization by Fluorescence Microscopy

Protein Localization by Fluorescence Microscopy
  • Author : Viki Allan
  • Publisher : OUP Oxford
  • Release Date : 1999-11-18
  • Total pages : 256
  • ISBN : 1489918663
GET BOOK

Summary : There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step ...

Fluorescence Microscopy

Fluorescence Microscopy
  • Author : B. Herman
  • Publisher : Garland Science
  • Release Date : 2020-08-27
  • Total pages : 212
  • ISBN : 1489918663
GET BOOK

Summary : This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems....

Fluorescence Microscopy

Fluorescence Microscopy
  • Author : Ulrich Kubitscheck
  • Publisher : John Wiley & Sons
  • Release Date : 2017-03-27
  • Total pages : 496
  • ISBN : 1489918663
GET BOOK

Summary : While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy ...

Cryogenic Super Resolved Fluorescence Microscopy

Cryogenic Super Resolved Fluorescence Microscopy
  • Author : Siegfried Weisenburger
  • Publisher : Logos Verlag Berlin GmbH
  • Release Date : 2016-11-16
  • Total pages : 173
  • ISBN : 1489918663
GET BOOK

Summary : The significance of super-resolved fluorescence microscopy beyond the diffraction barrier was recognized by the Nobel Prize in Chemistry in 2014. At room temperature, these techniques typically achieve a resolution on the order of twenty nanometers. They already allowed for resolving subcellular structures and organelles, and are starting to enable discoveries in ...